Mechanism of Action of Heme Oxygenase
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چکیده
The formation of bile pigment from heme by a reconstituted heme oxygenase system containing purified bovine spleen heme oxygenase, NADPH-cytochrome P-450 reductase, and biliverdin reductase was studied under an atmosphere containing '8.'802. The product, bilirubin, was isolated and subjected to mass spectrometry, which revealed incorporation of '*O consistent with a two-molecule mechanism, whereby the product bile pigment contains oxygen atoms derived from two different oxygen molecules.
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تاریخ انتشار 2001